Product Info Summary
SKU: | A10058 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IHC |
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Product info
Product Name
Anti-GUF1 Antibody
SKU/Catalog Number
A10058
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-GUF1 Antibody (Catalog # A10058). Tested in IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GUF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10058)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human GUF1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10058 is reactive to GUF1 in Human, Mouse
Applications
A10058 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
74.328kDa
Background of GUF1
Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner By similarity. HAMAP-Rule MF_03137
Ota T., Nat. Genet. 36:40-45(2004).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Vasilescu J., J. Proteome Res. 6:298-305(2007).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using GUF1 antibody A10058.
Protein Target Info & Infographic
Gene/Protein Information For GUF1 (Source: Uniprot.org, NCBI)
Gene Name
GUF1
Full Name
Translation factor GUF1, mitochondrial
Weight
74.328kDa
Superfamily
TRAFAC class translation factor GTPase superfamily
Alternative Names
EC 3.6.5.-; EF-4; Elongation factor 4 homolog; FLJ13220; GTPase GUF1; GTPase of unknown function 1; GTP-binding protein GUF1 homolog; GUF1 GTPase homolog (S. cerevisiae); Ribosomal back-translocase; translation factor GUF1, mitochondrial GUF1 DEE40, EF-4, EF4, EIEE40 GTP binding elongation factor GUF1 translation factor GUF1, mitochondrial|GTP-binding protein GUF1 homolog|GTPase of unknown function 1|GUF1 homolog, GTPase|elongation factor 4 homolog|ribosomal back-translocase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GUF1, check out the GUF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GUF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GUF1 Antibody (A10058)
Hello CJ!
No publications found for A10058
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-GUF1 Antibody
Question
We are currently using anti-GUF1 antibody A10058 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
The anti-GUF1 antibody (A10058) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon teratocarcinoma using anti-GUF1 antibody A10058. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-27
Question
My question regarding product A10058, anti-GUF1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10058 anti-GUF1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-02
Question
See attached the WB image, lot number and protocol we used for colon teratocarcinoma using anti-GUF1 antibody A10058. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-10-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-18
Question
Is this A10058 anti-GUF1 antibody reactive to the isotypes of GUF1?
Verified Customer
Verified customer
Asked: 2017-08-30
Answer
The immunogen of A10058 anti-GUF1 antibody is Synthesized peptide derived from internal of human GUF1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-08-30
Question
Will anti-GUF1 antibody A10058 work on monkey IHC with colon teratocarcinoma?
C. Evans
Verified customer
Asked: 2014-01-03
Answer
Our lab technicians have not validated anti-GUF1 antibody A10058 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-GUF1 antibody A10058 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey colon teratocarcinoma in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-01-03